He developed a dark field ultra microscope that could detect particles with diameters below the wavelengths of visible light. The uniform production of experimental shock by crush injury: Possible relationship to clinical crush syndrome. Ronald Aylmer Fisher (GB-AU) invented the statistical method known as analysis of variance (974). Abbott (US) developed a method for computing the effectiveness of insecticides (3). Hoffman (US) isolated adenine nucleotide in crystalline form from pig blood (1338). Cells that lacked membrane-bound cell nuclei, like bacteria and blue-green algae, were designated prokaryotes.
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Cyrus Hartwell Fiske (US) and Yellapragada Subbarow (US) developed a colorimetric method useful for the detection of phosphorus in organic material (980).
Evert Gorter (NL) and Francois Grendel (NL) determined that the area of the monomolecular film formed on a Langmuir trough by the membrane lipids was double the surface area of the erythrocytes from which the lipids had been extracted, suggesting that the cell membrane is bimolecular (1132).
They defined eukaryotes as cells containing membrane-bound structures called organelles, the most important of which was the nucleus. du Vigneaud V, Cohn M, Chandler JP, Schenck JR, Simmonds S. The utilization of the methyl group of methionine in the biological synthesis of choline and creatine.
By this definition, all cells of multi-cellular plants and animals were eukaryotes. The Journal of Biological Chemistry 140: 625-41 808.
Professor Emeritus of Biology Delta State University Box 3262, Cleveland, MS 38733 e-mail: [email protected]"The key to every biological problem must finally be sought in the cell." Edmund Beecher Wilson (3178).
Richard Adolf Zsigmondy (AT-DE) received the Nobel Prize in Chemistry for his demonstration of the heterogeneous nature of colloid solutions and for the methods he used, which have since become fundamental in modern colloid chemistry.
Emil-Karl Frey (DE) observed in 1925 a considerable reduction in arterial blood pressure when he injected human urine into dogs.
Unlike many other contemporary scientists he did not attribute this effect to a toxic action of urine, but rather as the specific activity of an unknown substance with potential biological functions (1020, 1021). Grunz (DE) discovered kinins when they gave evidence that a smooth muscle stimulating substance KLK (tissue kallikrein) is a proteolytic enzyme, which liberates the biologically highly active basic polypeptide kallidin (KD) from the plasma protein, kallidinogen or kininogen (3102, 3103).
Erwin Broun Fred (US), Ira Lawrence Baldwin (US) and Elizabeth Mc Coy (US) published the definitive text on nitrogen fixation (1012).